semi-quantitative analysis of hoxa11, leukemia inhibitory factor and basic transcriptional element binding protein 1 mrna expression in the mid-secretory endometrium of patients with endometriosis
نویسندگان
چکیده
background: hoxa11 and leukemia inhibitory factor (lif) and basic transcriptional element binding protein1 (bteb1) are expressed in endometrium throughout the menstrual cycle and show a dramatic increase during the mid-luteal phase at the time of implantation. in this case-control study, the expression pattern of these mrna was evaluated in patients with endometriosis at the time of implantation. we also describe a semi-quantitative rt-pcr protocol optimized in our laboratory. methods: eight patients with endometriosis were considered as our case and 8 fertile women as control group. expression levels of hoxa11, lif and bteb1 mrna were measured in endometrium during the mid-secretory phase using semi-quantitative rt-pcr. results: we describe the detailed procedure for the analysis of hoxa11, lif and bteb1 mrna levels. endometrial hoxa11 and lif mrna expression levels (normalized to ß-actin expression) were significantly decreased in endometrium of infertile patients with endometriosis compared with healthy fertile controls at the time of implantation (p<0.05). a similar trend was seen in bteb1 mrna expression. conclusion: the results suggest that the alteration in expression pattern of the some genes could account for some aspects of infertility in endometriosis.
منابع مشابه
Semi-Quantitative Analysis of HOXA11, Leukemia Inhibitory Factor and Basic Transcriptional Element Binding Protein 1 mRNA Expression in the Mid-Secretory Endometrium of patients with endometriosis
Background: HOXA11 and leukemia inhibitory factor (LIF) and basic transcriptional element binding protein1 (BTEB1) are expressed in endometrium throughout the menstrual cycle and show a dramatic increase during the mid-luteal phase at the time of implantation. In this case-control study, the expression pattern of these mRNA was evaluated in patients with endometriosis at the time of implantatio...
متن کاملSemi-quantitative analysis of HOXA11, leukemia inhibitory factor and basic transcriptional element binding protein 1 mRNA expression in the mid-secretory endometrium of patients with endometriosis.
BACKGROUND HOXA11 and leukemia inhibitory factor (LIF) and basic transcriptional element binding protein1 (BTEB1) are expressed in endometrium throughout the menstrual cycle and show a dramatic increase during the mid-luteal phase at the time of implantation. In this case-control study, the expression pattern of these mRNA was evaluated in patients with endometriosis at the time of implantation...
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the expression of the autophagy gene beclin-1 mrna and protein in ectopic and eutopic endometrium of patients with endometriosis
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متن کاملExpression of HOXA11 in the mid-luteal endometrium from women with endometriosis-associated infertility
BACKGROUND A decrease in HOXA11 expression in eutopic mid-secretory endometrium has been found in women with endometriosis-associated infertility. METHODS Using Real-time quantitative PCR (RQ-PCR) and western blotting analysis we studied the HOXA11 transcript and protein levels in mid-luteal eutopic endometrium from eighteen infertile women with minimal endometriosis, sixteen healthy fertile ...
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عنوان ژورنال:
iranian biomedical journalجلد ۱۵، شماره ۳، صفحات ۶۶-۷۲
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